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pgex4t 1 vector  (Danaher Inc)


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    Structured Review

    Danaher Inc pgex4t 1 vector
    Pgex4t 1 Vector, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pgex4t+1+vector/pmc11142376-280-19-21
    Average 86 stars, based on 1 article reviews
    pgex4t 1 vector - by Bioz Stars, 2026-09
    86/100 stars

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    Related Articles

    Amplification:

    Article Title: Dynamin is primed at endocytic sites for ultrafast endocytosis
    Article Snippet: .. The amplified product was inserted into pGEX4T-1 vector (Amersham Biosciences) using the restriction enzymes EcoRI and NotI (underlined in sequence). .. Subsequent modifications to generate S774/778E and S774/778A mutants used the QuickChange site-directed mutagenesis kit (Stratagene) and were confirmed by DNA sequencing ( ).

    Article Title: Dynamin is primed at endocytic sites for ultrafast endocytosis.
    Article Snippet: .. The amplified product was inserted into pGEX4T-1 vector (Amersham Biosciences) using the restriction enzymes EcoRI and NotI (underlined in sequence). .. Subsequent modifications to generate S774/778E and S774/778Amutants used the QuickChange site-directed mutagenesis kit (Stratagene) and were confirmed by DNA sequencing (Anggono et al., 2006).

    Article Title: Dynamin forms liquid-like condensates at synapses to support ultrafast endocytosis
    Article Snippet: .. The amplified product was inserted into pGEX4T-1 vector (Amersham Biosciences) using the restriction enzymes EcoRI and NotI (underlined in sequence). .. Subsequent modifications to generate S774/778E and S774/778A mutants used the QuickChange site-directed mutagenesis kit (Stratagene) and were confirmed by DNA sequencing ( ).

    Article Title: E3 SUMO ligase SIZ1 splicing variants localize and function according to external conditions
    Article Snippet: To generate His 6 -AtSUMO1-GG, His 6 -AtSUMO2-GG, His 6 -AtSUMO3-GG, and His 6 -AtSUMO5-GG, full-length cDNAs were amplified by PCR with gene-specific primers and cloned into the pET28a vector (Novagen). .. To produce glutathione S-transferase (GST)-CNGC6, full-length CNGC6 cDNA was amplified by PCR with gene-specific primers and cloned into the pGEX4T-1 vector (Amersham Biosciences). ..

    Plasmid Preparation:

    Article Title: Dynamin is primed at endocytic sites for ultrafast endocytosis
    Article Snippet: .. The amplified product was inserted into pGEX4T-1 vector (Amersham Biosciences) using the restriction enzymes EcoRI and NotI (underlined in sequence). .. Subsequent modifications to generate S774/778E and S774/778A mutants used the QuickChange site-directed mutagenesis kit (Stratagene) and were confirmed by DNA sequencing ( ).

    Article Title: Dynamin is primed at endocytic sites for ultrafast endocytosis.
    Article Snippet: .. The amplified product was inserted into pGEX4T-1 vector (Amersham Biosciences) using the restriction enzymes EcoRI and NotI (underlined in sequence). .. Subsequent modifications to generate S774/778E and S774/778Amutants used the QuickChange site-directed mutagenesis kit (Stratagene) and were confirmed by DNA sequencing (Anggono et al., 2006).

    Article Title: Dynamin forms liquid-like condensates at synapses to support ultrafast endocytosis
    Article Snippet: .. The amplified product was inserted into pGEX4T-1 vector (Amersham Biosciences) using the restriction enzymes EcoRI and NotI (underlined in sequence). .. Subsequent modifications to generate S774/778E and S774/778A mutants used the QuickChange site-directed mutagenesis kit (Stratagene) and were confirmed by DNA sequencing ( ).

    Article Title: Heterozygous Variants in MYBPC1 are Associated with an Expanded Neuromuscular Phenotype beyond Arthrogryposis
    Article Snippet: .. The PCR fragment was subcloned into the pGex4T-1 vector (Amersham Pharmacia, Piscataway, NJ, USA) at Eco RI/ Xho I sites to generate a GST-fusion protein. ..

    Article Title: Phosphoproteomic analysis reveals that dehydrins ERD10 and ERD14 are phosphorylated by SNF1-related protein kinase 2.10 in response to osmotic stress.
    Article Snippet: .. Full-length cDNAs for ERD10 and ERD14 were PCR-amplified using specific primers (listed in Supplemental Table 5) and cloned as EcoRI/SalI fragments into a pGEX4T-1 vector (Amersham Biosciences). .. All PCR reactions were performed using highfidelity Phusion polymerase (Thermo Fisher Scientific) and verified by DNA sequencing.

    Article Title: The Mitochondrial Protein MAVS Stabilizes p53 to Suppress Tumorigenesis.
    Article Snippet: .. Plasmids encoding glutathione S-transferase (GST) fusion proteins were prepared by cloning PCR-amplified fragments into a pGEX4T-1 vector (Amersham Biosciences). ..

    Article Title: E3 SUMO ligase SIZ1 splicing variants localize and function according to external conditions
    Article Snippet: To generate His 6 -AtSUMO1-GG, His 6 -AtSUMO2-GG, His 6 -AtSUMO3-GG, and His 6 -AtSUMO5-GG, full-length cDNAs were amplified by PCR with gene-specific primers and cloned into the pET28a vector (Novagen). .. To produce glutathione S-transferase (GST)-CNGC6, full-length CNGC6 cDNA was amplified by PCR with gene-specific primers and cloned into the pGEX4T-1 vector (Amersham Biosciences). ..

    Sequencing:

    Article Title: Dynamin is primed at endocytic sites for ultrafast endocytosis
    Article Snippet: .. The amplified product was inserted into pGEX4T-1 vector (Amersham Biosciences) using the restriction enzymes EcoRI and NotI (underlined in sequence). .. Subsequent modifications to generate S774/778E and S774/778A mutants used the QuickChange site-directed mutagenesis kit (Stratagene) and were confirmed by DNA sequencing ( ).

    Article Title: Dynamin is primed at endocytic sites for ultrafast endocytosis.
    Article Snippet: .. The amplified product was inserted into pGEX4T-1 vector (Amersham Biosciences) using the restriction enzymes EcoRI and NotI (underlined in sequence). .. Subsequent modifications to generate S774/778E and S774/778Amutants used the QuickChange site-directed mutagenesis kit (Stratagene) and were confirmed by DNA sequencing (Anggono et al., 2006).

    Article Title: Dynamin forms liquid-like condensates at synapses to support ultrafast endocytosis
    Article Snippet: .. The amplified product was inserted into pGEX4T-1 vector (Amersham Biosciences) using the restriction enzymes EcoRI and NotI (underlined in sequence). .. Subsequent modifications to generate S774/778E and S774/778A mutants used the QuickChange site-directed mutagenesis kit (Stratagene) and were confirmed by DNA sequencing ( ).

    Polymerase Chain Reaction:

    Article Title: Heterozygous Variants in MYBPC1 are Associated with an Expanded Neuromuscular Phenotype beyond Arthrogryposis
    Article Snippet: .. The PCR fragment was subcloned into the pGex4T-1 vector (Amersham Pharmacia, Piscataway, NJ, USA) at Eco RI/ Xho I sites to generate a GST-fusion protein. ..

    Article Title: Phosphoproteomic analysis reveals that dehydrins ERD10 and ERD14 are phosphorylated by SNF1-related protein kinase 2.10 in response to osmotic stress.
    Article Snippet: .. Full-length cDNAs for ERD10 and ERD14 were PCR-amplified using specific primers (listed in Supplemental Table 5) and cloned as EcoRI/SalI fragments into a pGEX4T-1 vector (Amersham Biosciences). .. All PCR reactions were performed using highfidelity Phusion polymerase (Thermo Fisher Scientific) and verified by DNA sequencing.

    Article Title: The Mitochondrial Protein MAVS Stabilizes p53 to Suppress Tumorigenesis.
    Article Snippet: .. Plasmids encoding glutathione S-transferase (GST) fusion proteins were prepared by cloning PCR-amplified fragments into a pGEX4T-1 vector (Amersham Biosciences). ..

    Article Title: E3 SUMO ligase SIZ1 splicing variants localize and function according to external conditions
    Article Snippet: To generate His 6 -AtSUMO1-GG, His 6 -AtSUMO2-GG, His 6 -AtSUMO3-GG, and His 6 -AtSUMO5-GG, full-length cDNAs were amplified by PCR with gene-specific primers and cloned into the pET28a vector (Novagen). .. To produce glutathione S-transferase (GST)-CNGC6, full-length CNGC6 cDNA was amplified by PCR with gene-specific primers and cloned into the pGEX4T-1 vector (Amersham Biosciences). ..

    Clone Assay:

    Article Title: Phosphoproteomic analysis reveals that dehydrins ERD10 and ERD14 are phosphorylated by SNF1-related protein kinase 2.10 in response to osmotic stress.
    Article Snippet: .. Full-length cDNAs for ERD10 and ERD14 were PCR-amplified using specific primers (listed in Supplemental Table 5) and cloned as EcoRI/SalI fragments into a pGEX4T-1 vector (Amersham Biosciences). .. All PCR reactions were performed using highfidelity Phusion polymerase (Thermo Fisher Scientific) and verified by DNA sequencing.

    Article Title: E3 SUMO ligase SIZ1 splicing variants localize and function according to external conditions
    Article Snippet: To generate His 6 -AtSUMO1-GG, His 6 -AtSUMO2-GG, His 6 -AtSUMO3-GG, and His 6 -AtSUMO5-GG, full-length cDNAs were amplified by PCR with gene-specific primers and cloned into the pET28a vector (Novagen). .. To produce glutathione S-transferase (GST)-CNGC6, full-length CNGC6 cDNA was amplified by PCR with gene-specific primers and cloned into the pGEX4T-1 vector (Amersham Biosciences). ..

    Cloning:

    Article Title: The Mitochondrial Protein MAVS Stabilizes p53 to Suppress Tumorigenesis.
    Article Snippet: .. Plasmids encoding glutathione S-transferase (GST) fusion proteins were prepared by cloning PCR-amplified fragments into a pGEX4T-1 vector (Amersham Biosciences). ..



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